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KMID : 0379119940220030276
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Korean Journal of Mycology
1994 Volume.22 No. 3 p.276 ~ p.280
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A Efficient Selection of Hybrids Following Intergeneric Transfer of Nuclei from Trichoderma harzianum into Gliocladium virens Protoplasts
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류진창/Ryu, Jin Chang
조무제/신평균/유영복/박용환/Cho, Moo Je/Shin, Pyung Gyun/Yoo, Young Bok/Park, Young Hwan
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Abstract
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To obtain hybrids producing antagouisms and plant growth promoting effects by intergeneric nuclei transfer, the nuclei were isolated from the protoplasts of Trichoderma harzianum T95 and treated with colchicine. The nuclei were transferred into protoplast of multi-auxotrophic Gllecladium virens G88 which cannot grow is minimal medium. The nuclei transferred into protoplasts of G. virens G88 were selected on the regeneration minimal medium containing chloroneb as however no segregants were found in the intergeneric unclei transfer. The various types of hybrids with different morphology were detected when different concentration of chloroneb were treated. These morphologies were classified as parental, recombinant and petite type.
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